Composite

Part:BBa_K4757063:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-12)


pEM7-XylS-K38R-L224Q/Pm expression cassete


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 111
    Illegal BamHI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 833
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

high leaky expression. The transcription factor XylS is encoded on the plasmid vector in reverse complement.


Source

XylS/Pm originiated from the TOL plasmid from Pseudomonas putida. pEM7 is a synthetic promoter

References