Composite
Part:BBa_K4757063:Design
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-10-12)
pEM7-XylS-K38R-L224Q/Pm expression cassete
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 111
Illegal BamHI site found at 61 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 833
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
high leaky expression. The transcription factor XylS is encoded on the plasmid vector in reverse complement.
Source
XylS/Pm originiated from the TOL plasmid from Pseudomonas putida. pEM7 is a synthetic promoter